LOCATION AND RESOURCES

The MC@CHOP is located on the fifth floor of the Abramson Research Center (ARC) Building.This facility includes a 2200 square foot laboratory space where equipment is located and analyses are performed. All necessary fume hoods and bio-safety cabinets are part of the core. PC Computers, iMacs and MAC-Books are available for the staff. The computers are connected to the institutional database and used for data medium, data analyses and storage.

Our GC-MS instruments include 3 Hewlett-Packard/Agilent GC-MS, each with an automated sampling system that permits the analysis of up to 99 unattended samples. Each system is controlled by an individual computer/work station with Windows-based proprietary software from HP/Agilent. These instruments are used primarily for the analysis of 15N, 2H or 13C in glucose, amino acids, fatty acids, primary amines and organic acids (particularly tricarboxylic acid cycle intermediates such as citrate, ketoglutarate, succinate, malate).

Our isotope ratio-mass spectrometry is utilized for the analysis of 13CO2 in metabolic studies as well as the analysis of 2H2O in doubly-labelled water studies to measure the in vivo metabolic rate of fatty acid oxidation and glycolysis. Instruments utilized to this end include Thermo Finnigan DeltaPLUS continuous flow and Thermo DELTA V Advantage IRMS, equipped with an autosampler that permits unattended analysis of 96 samples. Sensitivity of the latter instrument (atom % excess) characteristically exceeds 10-5.

We also maintain 2 LC-MS triple stage quadrupole systems. One is a VG Quattro II LC-MS unit and the other is an Agilent 6400 LC-triple stage quadrupole system. The latter is used to perform metabolomic research, or the profiling in a single sample of > 100 individual metabolites in order to create a metabolic "fingerprint."

HPLC instruments include Agilent-1260 Infinity HPLC and two Varian HPLC systems equipped with UV and fluorescent detectors, and are used to measure metabolite levels as an integral part of stable isotope analysis and determination of flux rates.

For monitoring of enzyme assays, we have an Enspire 2300 multilabeled Reader, and other spectrophotometers used for enzymatic assays.

The core is equipped with systems to monitor oxygen consumption in isolated mitochondria or cells, whole organ (liver, kidney, heart), perfusion systems as well as all necessary centrifuges, balances, pH meters, metabolic incubators, cold room, refrigerators and freezers for sample preparation and storage.